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Brilliant Blue 660-P2

BB660-P (Ex-Max 484 nm/Em-Max 695 nm)

Horizon Brilliant™ Blue 660-P (BB660-P) from BD Biosciences is a tandem dye of BB515. It has an excitation peak at 484 nm and an emission peak at 667 nm, making it spectrally comparable to PE-Cy5.

Excitation & emission spectra

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Key facts

Excitation maximum
484 nm
Emission maximum
667 nm
Optimal laser line
488
Optimal filter
660/20
Relative brightness
3 of 5

Brilliant Blue 660-P2 has an excitation maximum at 484 nm and an emission maximum at 667 nm, a Stokes shift of 183 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is Brilliant Blue 660-P2?
Horizon Brilliant™ Blue 660-P (BB660-P) from BD Biosciences is a tandem dye of BB515. It has an excitation peak at 484 nm and an emission peak at 667 nm, making it spectrally comparable to PE-Cy5.
What laser excites Brilliant Blue 660-P2?
Brilliant Blue 660-P2 is optimally excited by the 488 laser line. Its excitation maximum is 484 nm.
What filter should I use to detect Brilliant Blue 660-P2?
A 660/20 filter captures the peak of the Brilliant Blue 660-P2 emission near 667 nm with minimal spillover.
How bright is Brilliant Blue 660-P2?
Brilliant Blue 660-P2 has a relative brightness of 3 out of 5.