NovaFluor Blue 530
NovaFluor Blue 530 (Ex-Max 509 nm/Em-Max 530 nm)
The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Blue 530 is designed to be discreetly excited by the 488 nm laser and has an excitation peak at 509 nm and an emission peak at 530 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 509 nm
- Emission maximum
- 530 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Relative brightness
- 1 of 5
- Commercial antibodies
- 23 from 1 supplier
NovaFluor Blue 530 has an excitation maximum at 509 nm and an emission maximum at 530 nm, a Stokes shift of 21 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is NovaFluor Blue 530?
- The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Blue 530 is designed to be discreetly excited by the 488 nm laser and has an excitation peak at 509 nm and an emission peak at 530 nm.
- What laser excites NovaFluor Blue 530?
- NovaFluor Blue 530 is optimally excited by the 488 laser line. Its excitation maximum is 509 nm.
- What filter should I use to detect NovaFluor Blue 530?
- A 525/50 filter captures the peak of the NovaFluor Blue 530 emission near 530 nm with minimal spillover.
- How bright is NovaFluor Blue 530?
- NovaFluor Blue 530 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to NovaFluor Blue 530?
- FluoroFinder tracks 23 commercially available NovaFluor Blue 530 conjugates, searchable by target, clone, host and application.