SureLight APC
SureLight APC (Ex-Max 650 nm/Em-Max 660 nm)
SureLight APC from Columbia Biosciences is a fluorescent protein with an excitation peak at 650 nm and an emission peak at 660 nm. SureLight APC is available in antibody conjugation kits for use in flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 650 nm
- Emission maximum
- 660 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 5 of 5
- Extinction coefficient
- 750000
- Molecular weight
- 105000
- Commercial antibodies
- 12 from 2 suppliers
SureLight APC has an excitation maximum at 650 nm and an emission maximum at 660 nm, a Stokes shift of 10 nm. Its extinction coefficient is 750000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is SureLight APC?
- SureLight APC from Columbia Biosciences is a fluorescent protein with an excitation peak at 650 nm and an emission peak at 660 nm. SureLight APC is available in antibody conjugation kits for use in flow cytometry.
- What laser excites SureLight APC?
- SureLight APC is optimally excited by the 628-640 laser line. Its excitation maximum is 650 nm.
- What filter should I use to detect SureLight APC?
- A 660/20 filter captures the peak of the SureLight APC emission near 660 nm with minimal spillover.
- How bright is SureLight APC?
- SureLight APC has a relative brightness of 5 out of 5.
- How many antibodies are available conjugated to SureLight APC?
- FluoroFinder tracks 12 commercially available SureLight APC conjugates, searchable by target, clone, host and application.