About Aldefluor

ALDeFluor™ is a non-immunological fluorescent reagent system for the detection of aldehyde dehydrogenase-bright (ALDHbr) in living cells. High expression of ALDH has been reported for normal and cancer stem and progenitor cells of various lineages, including hematopoietic, mammary, endothelial, mesenchymal and neural cells. It has an excitation peak at 488 nm and an emission peak at 512 nm. The ALDeFluor™ kit contains BODIPY-aminoacetaldehyde (BAAA), a fluorescent non-toxic substrate for ALDH, which freely diffuses into intact and viable cells. In the presence of ALDH, BAAA is converted into BODIPY-aminoacetate (BAA), a negatively charged product that is retained inside the cells. Intracellular accumulation of BAA leads to increased fluorescence, and these ALDH-bright (ALDHbr) cells can be analyzed with flow cytometry.