Sytox Orange
Sytox Orange (Ex-Max 546 nm/Em-Max 570 nm)
Sytox Orange from Thermo Fisher Scientific is a cell-impermeant, fluorogenic nucleic acid stain that can be used as a viability dye for use in flow cytometry and as a nuclear counterstain in fluorescence microscopy. It has an excitation peak at 546 nm and an emission peak at 570 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 546 nm
- Emission maximum
- 570 nm
- Optimal laser line
- 488-532
- Optimal filter
- 585/40
- Commercial antibodies
- 1 from 1 supplier
Sytox Orange has an excitation maximum at 546 nm and an emission maximum at 570 nm, a Stokes shift of 24 nm. It is optimally excited by the 488-532 laser line.
Frequently asked questions
- What is Sytox Orange?
- Sytox Orange from Thermo Fisher Scientific is a cell-impermeant, fluorogenic nucleic acid stain that can be used as a viability dye for use in flow cytometry and as a nuclear counterstain in fluorescence microscopy. It has an excitation peak at 546 nm and an emission peak at 570 nm.
- What laser excites Sytox Orange?
- Sytox Orange is optimally excited by the 488-532 laser line. Its excitation maximum is 546 nm.
- What filter should I use to detect Sytox Orange?
- A 585/40 filter captures the peak of the Sytox Orange emission near 570 nm with minimal spillover.
- How many antibodies are available conjugated to Sytox Orange?
- FluoroFinder tracks 1 commercially available Sytox Orange conjugates, searchable by target, clone, host and application.