Spectrum Green
Spectrum Green (Ex-Max 495 nm/Em-Max 519 nm)
SpectrumGreen™ is a fluorophore from Vysis with an excitation peak at 497 nm and an emission peak at 524 nm. The Vysis Spectrum family of fluorophores are designed for use in nucleotide labeling and FISH (Fluorescence In Situ Hydbridization) probes.
Excitation & emission spectra
Key facts
- Excitation maximum
- 495 nm
- Emission maximum
- 519 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Relative brightness
- 2 of 5
Spectrum Green has an excitation maximum at 495 nm and an emission maximum at 519 nm, a Stokes shift of 24 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Spectrum Green?
- SpectrumGreen™ is a fluorophore from Vysis with an excitation peak at 497 nm and an emission peak at 524 nm. The Vysis Spectrum family of fluorophores are designed for use in nucleotide labeling and FISH (Fluorescence In Situ Hydbridization) probes.
- What laser excites Spectrum Green?
- Spectrum Green is optimally excited by the 488 laser line. Its excitation maximum is 495 nm.
- What filter should I use to detect Spectrum Green?
- A 515/30 filter captures the peak of the Spectrum Green emission near 519 nm with minimal spillover.
- How bright is Spectrum Green?
- Spectrum Green has a relative brightness of 2 out of 5.